Reflex a-Adrenergic Coronary Vasoconstriction during Hindlimb Static Exercise in Dogs

We studied 18 a-chloralose-anesthetized dogs to determine if a-adrenergic coronary vasoconstriction occurs with hindlimb static exercise. Exercise was elicited by spinal cord ventral nerve root stimulation. Regional coronary blood flow was determined by the radioactive microsphere method. Animals were studied under four experimental conditions: control rest and static exercise, rest and static exercise after /?-adrenergic blockade with propranolol (2 mg/kg), rest and exercise after a-adrenergic blockade with phentolamine (.35 mg/kg), and rest and exercise after combined aand /?adrenergic blockade. Myocardial oxygen consumption during exercise was determined during control and during a-adrenergic blockade conditions. Control hindlimb static exercise resulted in significant increases in systolic (10.6%) and diastolic (12.5%) arterial pressures, heart rate (12.2%), and double product (24.6%). Associated with the increased demand for oxygen, myocardial oxygen consumption increased (33.6%) as did left ventricular myocardial flow (29.6%). However, left ventricular coronary vascular resistance was unchanged during static exercise. After /?-adrenergic blockade, systolic (12.2%) and diastolic (11.6%) arterial pressures and double product (10.7%) still increased significantly, but heart rate did not change with static exercise. In contrast, a-adrenergic vasoconstriction was unmasked as left (LV) and right (RV) ventricular myocardial blood flow decreased (LV: -30.0%, RV: -25.0%) and coronary vascular resistance increased (LV: 52.5%, RV: 45.3%) with static exercise. Combined aand y3-adrenergic blockade abolished the reduction in myocardial blood flow and the increase in coronary vascular resistance which occurred with static exercise after /3-adrenergic blockade. These data suggest that, during static exercise, reflexes from skeletal muscles can cause a-adrenergic coronary artery vasoconstriction. Circ Res 48: 502-509, 1981